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EXP-#649Lab Protocol Datasheet
🧬 UnspecifiedHusbandry

Manual marble-burying scoring (a single end-point marble count) is highly variable between observers and discards the temporal/behavioral detail needed for reliable cross-lab comparison.

The hack / solution

Standardize the marble-burying test to improve reproducibility: 1) Use a 26.6 x 42.5 x 18.5 cm cage with bedding roughly 4 cm deep. 2) Arrange 20 blue glass marbles in 4 evenly spaced rows. 3) Run a 30-minute session recorded on video at 25 fps rather than relying on a single end-of-session count. 4) Score recordings with a machine-learning classifier (e.g. JAABA) to quantify individual burying bouts, bout duration, and spatial heatmaps instead of just a final marble tally, which better distinguishes genuine burying behavior from incidental marble displacement.

📎Source: A Novel Automated Approach for Improving Standardization of the Marble Burying Test — PMC (PMC8982638)🔗View source🗓️Added: 2026-07-16Reviewed by: MouseStuff Admin
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