Hey everyone, We've been getting really low DNA yield from our tail snip lysis prep lately — Nanodrop readings are coming back well below what we'd normally expect, and a decent chunk of our genotyping PCRs are failing outright, presumably because there's just not enough template to amplify. Our current protocol is a 2-hour digestion at 55C with Proteinase K, which is what we've always done, so I'm not sure what changed.

The hack / solution

We switched our protocol to an overnight digestion (12-16 hrs) at 55C using freshly aliquoted Proteinase K, and our yields went up dramatically — usually 3-5x higher than what we were getting with the 2-hour digest. Update: switched to overnight digestion with a freshly ordered batch of Proteinase K and our yields are back to normal (actually better than before).

📎Source: genotyping_low_yield_noise.txt

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