Hi all, Wanted to flag an issue we've been chasing down for a couple weeks in case other labs sharing our genotyping bench have run into it too. We started seeing faint but unmistakable bands in our no-template (water) control lanes for our Cre genotyping PCR.

The hack / solution

Two changes fixed it completely for us: 1. Switched to filter (aerosol-barrier) pipette tips for all genotyping PCR setup, especially for master mix and template addition steps. Curious if it fixed the ramp rate issue people were complaining about.

📎Source: genotyping_contamination_noise.txt

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